Työssä tutkittujen sienien nimistä: Sienien nimestä.
- AMANITA-laji, Kärpässienilaji
A. phalloides , Kavala kärpässieni, Lömsk flugsvamp
- LEPIOTA-lajeja , suomukaslajeja, Fjällskivlingar
Euroopassa on n.50 suomukaslajia ja niistä usea on myrkyllinen.
Lepiota cristata, Syrlig fjällskivling
Lepiota brunneoincarnata
- INOCYBE-lajeja, Risakaslajeja, Risakkaita, Trådskivlingar, Trådingar
Sitaatti tiivistelmästä (engl.).
Abstract
Amanita phalloides,
Lepiota cristata, Lepiota brunneoincarnata and Inocybe asterospora are
among the most important species responsible of mushroom poisoning in
northern Italy. A real time PCR method for the identification of samples
containing DNA from each of these species was developed. To test
specificity all protocols were applied on DNA extracted from various
mushroom species; sensitivity was assessed performing serial dilutions
on all samples; versatility of the protocols was evaluated performing
tests on DNA extracted from different matrices. The protocols showed
high sensitivity (32 ng dried mushroom), high specificity and sensitive
detection of DNA extracted from difficult samples, including pasta with
mushroom, cooked mushrooms and gastric aspirates.
- PMID:
- 20524605
- [PubMed - indexed for MEDLINE]
-
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