P-Italian merkittävimmät myrkkysienet sienisuvuista AMANITA, LEPIOTA, INOCYBE

Mycologia. 2010 May-Jun;102(3):747-54. 
 http://www.ncbi.nlm.nih.gov/pubmed/20524605
 Molecular detection of poisonous mushrooms in different matrices.

Epis S, Matinato C, Gentili G, Varotto F, Bandi C, Sassera D.Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università degli studi di Milano, Milano, Italy.

Työssä tutkittujen sienien nimistä: Sienien nimestä.

• AMANITA-laji, Kärpässienilaji

A. phalloides , Kavala kärpässieni, Lömsk flugsvamp

• LEPIOTA-lajeja , suomukaslajeja, Fjällskivlingar

Euroopassa on n.50 suomukaslajia ja niistä usea on myrkyllinen.
Lepiota cristata, Syrlig fjällskivling
Lepiota brunneoincarnata

• INOCYBE-lajeja, Risakaslajeja, Risakkaita, Trådskivlingar, Trådingar 

Sitaatti  tiivistelmästä (engl.).

Abstract

Amanita phalloides, Lepiota cristata, Lepiota brunneoincarnata and Inocybe asterospora are among the most important species responsible of mushroom poisoning in northern Italy. A real time PCR method for the identification of samples containing DNA from each of these species was developed. To test specificity all protocols were applied on DNA extracted from various mushroom species; sensitivity was assessed performing serial dilutions on all samples; versatility of the protocols was evaluated performing tests on DNA extracted from different matrices. The protocols showed high sensitivity (32 ng dried mushroom), high specificity and sensitive detection of DNA extracted from difficult samples, including pasta with mushroom, cooked mushrooms and gastric aspirates.

PMID:

20524605

[PubMed - indexed for MEDLINE]

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